7XX2

Cryo-EM structure of Sr35 resistosome induced by AvrSr35 R381A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E, F (A, C, E, G, I)	CNL9	polymer	929	105392.2	5	UniProt (S5ABD6) Pfam (PF18052) Pfam (PF00931)	Triticum monococcum
2	B (B)	AvrSr35	polymer	575	66019.5	1	UniProt (A0A5B0N367)	Puccinia graminis f. sp. tritici
3	G, H, I, J, K (A, C, E, G, I)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	5	Chemie (ATP)

Sequence modifications

A, C, E, G, I: 1 - 919 (UniProt: S5ABD6)

PDB	External Database	Details
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B: 26 - 578 (UniProt: A0A5B0N367)

PDB	External Database	Details
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Gly 12	-	expression tag
Val 13	-	expression tag
Asp 14	-	expression tag
Leu 15	-	expression tag
Gly 16	-	expression tag
Thr 17	-	expression tag
Glu 18	-	expression tag
Asn 19	-	expression tag
Leu 20	-	expression tag
Tyr 21	-	expression tag
Phe 22	-	expression tag
Gln 23	-	expression tag
Ser 24	-	expression tag
Asn 25	-	expression tag
Ala 381	Arg 381	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	592980.5
Non-Polymers*	Number of molecules	5
	Total formula weight	2535.9
All*	Total formula weight	595516.4

*Water molecules are not included.