7XUR

The cryo-EM structure of human mini-SNAPc in complex with hU6-1 PSE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	snRNA-activating protein complex subunit 4	polymer	522	60780.3	1	UniProt (Q5SXM2) Pfam (PF13921) In PDB	Homo sapiens (human)	SNAPc subunit 4,Proximal sequence element-binding transcription factor subunit alpha,PSE-binding factor subunit alpha,PTF subunit alpha,snRNA-activating protein complex 190 kDa subunit,SNAPc 190 kDa subunit
2	B	snRNA-activating protein complex subunit 3	polymer	411	46812.6	1	UniProt (Q92966) Pfam (PF12251) In PDB	Homo sapiens (human)	SNAPc subunit 3,Proximal sequence element-binding transcription factor subunit beta,PSE-binding factor subunit beta,PTF subunit beta,Small nuclear RNA-activating complex polypeptide 3,snRNA-activating protein complex 50 kDa subunit,SNAPc 50 kDa subunit
3	C	snRNA-activating protein complex subunit 1	polymer	282	33559.5	1	UniProt (Q16533) Pfam (PF09808) In PDB	Homo sapiens (human)	SNAPc subunit 1,Proximal sequence element-binding transcription factor subunit gamma,PSE-binding factor subunit gamma,PTF subunit gamma,Small nuclear RNA-activating complex polypeptide 1,snRNA-activating protein complex 43 kDa subunit,SNAPc 43 kDa subunit
4	X	DNA (35-MER)	polymer	35	10726.9	1		synthetic construct
5	Y	DNA (35-MER)	polymer	35	10803.0	1		synthetic construct
6	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 505 (UniProt: Q5SXM2)

PDB	External Database	Details
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Ser -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

C: 1 - 268 (UniProt: Q16533)

PDB	External Database	Details
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	162682.3
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	162813.1

*Water molecules are not included.