7XUE
Cryo-EM structure of HK022 putRNA-associated E.coli RNA polymerase elongation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | non-template DNA | polymer | 177 | 54757.0 | 1 | Shamshuipovirus HK022 | ||
| 2 | B (B) | template DNA | polymer | 177 | 54512.9 | 1 | Shamshuipovirus HK022 | ||
| 3 | C (R) | RNA (nun gene and immunity region) | polymer | 95 | 30714.2 | 1 | GenBank (435309) | Shamshuipovirus HK022 | |
| 4 | D, E (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli (strain K12) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | F (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli (strain K12) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | G (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1430 | 158073.6 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli (strain K12) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | H (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli (strain K12) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | I, J (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 9 | K (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
J: 1 - 1407 (UniProt: P0A8T7)
| PDB | External Database | Details |
|---|---|---|
| Val 1 | Met 1 | conflict |
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Leu 1410 | - | expression tag |
| Glu 1411 | - | expression tag |
| Val 1412 | - | expression tag |
| Leu 1413 | - | expression tag |
| Phe 1414 | - | expression tag |
| Gln 1415 | - | expression tag |
| Gly 1416 | - | expression tag |
| Pro 1417 | - | expression tag |
| Ser 1418 | - | expression tag |
| Ser 1419 | - | expression tag |
| Gly 1420 | - | expression tag |
| His 1421 | - | expression tag |
| His 1422 | - | expression tag |
| His 1423 | - | expression tag |
| His 1424 | - | expression tag |
| His 1425 | - | expression tag |
| His 1426 | - | expression tag |
| His 1427 | - | expression tag |
| His 1428 | - | expression tag |
| His 1429 | - | expression tag |
| His 1430 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 532245.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 532400.6 |
