7XTB

Serotonin 6 (5-HT6) receptor-Gs-Nb35 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody35	polymer	139	15271.9	1		Lama glama
5	E (R)	Soluble cytochrome b562,5-hydroxytryptamine receptor 6	polymer	584	63410.2	1	UniProt (P0ABE7) UniProt (P50406) Pfam (PF07361) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562,5-HT-6,5-HT6,Serotonin receptor 6
6	F (R)	SEROTONIN	non-polymer		176.2	1	Chemie (SRO)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 226	conflict
Ala 268	Glu 268	conflict
Lys 271	Asn 271	conflict
Asp 274	Lys 274	conflict
Lys 280	Arg 280	conflict
Asp 284	Thr 284	conflict
Thr 285	Ile 285	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -143	-	expression tag
Tyr -142	-	expression tag
Lys -141	-	expression tag
Asp -140	-	expression tag
Asp -139	-	expression tag
Asp -138	-	expression tag
Asp -137	-	expression tag
Ala -136	-	expression tag
Lys -135	-	expression tag
Leu -134	-	expression tag
Gln -133	-	expression tag
Thr -132	-	expression tag
Met -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Ala -9	-	linker
Ser -8	-	linker
Glu -7	-	linker
Asn -6	-	linker
Leu -5	-	linker
Tyr -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Gly 0	-	linker
Thr 1	-	linker

R: 2 - 440 (UniProt: P50406)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	170142.2
Non-Polymers*	Number of molecules	1
	Total formula weight	176.2
All*	Total formula weight	170318.4

*Water molecules are not included.