7XT8
Serotonin 4 (5-HT4) receptor-Gs-Nb35 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
| 4 | N | Nanobody35 | polymer | 139 | 15271.9 | 1 | Lama glama | ||
| 5 | R | Soluble cytochrome b562,5-hydroxytryptamine receptor 4 | polymer | 527 | 59525.6 | 1 | UniProt (P0ABE7) UniProt (Q13639) Pfam (PF07361) Pfam (PF00001) In PDB | Homo sapiens (human) | Cytochrome b-562,5-HT-4,5-HT4,Serotonin receptor 4 |
| 6 | R | CHOLESTEROL | non-polymer | 386.7 | 5 | Chemie (CLR) | |||
| 7 | R | PALMITIC ACID | non-polymer | 256.4 | 2 | Chemie (PLM) | |||
| 8 | R | SEROTONIN | non-polymer | 176.2 | 1 | Chemie (SRO) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: -116 - -12 (UniProt: P0ABE7)
R: 1 - 387 (UniProt: Q13639)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | conflict |
| Ala 226 | Gly 226 | conflict |
| Ala 268 | Glu 268 | conflict |
| Lys 271 | Asn 271 | conflict |
| Asp 274 | Lys 274 | conflict |
| Lys 280 | Arg 280 | conflict |
| Asp 284 | Thr 284 | conflict |
| Thr 285 | Ile 285 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -139 | - | expression tag |
| Tyr -138 | - | expression tag |
| Lys -137 | - | expression tag |
| Asp -136 | - | expression tag |
| Asp -135 | - | expression tag |
| Asp -134 | - | expression tag |
| Asp -133 | - | expression tag |
| Ala -132 | - | expression tag |
| Lys -131 | - | expression tag |
| Leu -130 | - | expression tag |
| Gln -129 | - | expression tag |
| Thr -128 | - | expression tag |
| Met -127 | - | expression tag |
| His -126 | - | expression tag |
| His -125 | - | expression tag |
| His -124 | - | expression tag |
| His -123 | - | expression tag |
| His -122 | - | expression tag |
| His -121 | - | expression tag |
| His -120 | - | expression tag |
| His -119 | - | expression tag |
| His -118 | - | expression tag |
| His -117 | - | expression tag |
| Trp -110 | Met 29 | conflict |
| Ile -15 | His 124 | conflict |
| Leu -11 | - | linker |
| Ala -10 | - | linker |
| Ser -9 | - | linker |
| Glu -8 | - | linker |
| Asn -7 | - | linker |
| Leu -6 | - | linker |
| Tyr -5 | - | linker |
| Phe -4 | - | linker |
| Gln -3 | - | linker |
| Gly -2 | - | linker |
| Gly -1 | - | linker |
| Thr 0 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 166257.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2622.3 | |
| All* | Total formula weight | 168879.9 |
