7XP4

Cryo-EM structure of a class T GPCR in apo state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(t) subunit alpha-3	polymer	264	30583.3	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	139	15271.9	1		Homo sapiens
5	E (R)	Endoglucanase H,Taste receptor type 2 member 46,Endoglucanase H,Taste receptor type 2 member 46,Bitter taste receptor T2R46	polymer	802	91116.3	1	UniProt (H6SHY4) UniProt (P59540) Pfam (PF02156) Pfam (PF05296) UniProt (by SIFTS) (P16218)	Acetivibrio thermocellus	T2R46,Taste receptor type 2 member 54,T2R54

Sequence modifications

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -277 - 1 (UniProt: H6SHY4)

PDB	External Database	Details
Met -324	-	initiating methionine
Lys -323	-	expression tag
Thr -322	-	expression tag
Ile -321	-	expression tag
Ile -320	-	expression tag
Ala -319	-	expression tag
Leu -318	-	expression tag
Ser -317	-	expression tag
Tyr -316	-	expression tag
Ile -315	-	expression tag
Phe -314	-	expression tag
Cys -313	-	expression tag
Leu -312	-	expression tag
Val -311	-	expression tag
Phe -310	-	expression tag
Ala -309	-	expression tag
Asp -308	-	expression tag
Tyr -307	-	expression tag
Lys -306	-	expression tag
Asp -305	-	expression tag
Asp -304	-	expression tag
Asp -303	-	expression tag
Asp -302	-	expression tag
Ala -301	-	expression tag
His -300	-	expression tag
His -299	-	expression tag
His -298	-	expression tag
His -297	-	expression tag
His -296	-	expression tag
His -295	-	expression tag
His -294	-	expression tag
His -293	-	expression tag
His -292	-	expression tag
His -291	-	expression tag
Glu -290	-	expression tag
Asn -289	-	expression tag
Leu -288	-	expression tag
Tyr -287	-	expression tag
Phe -286	-	expression tag
Gln -285	-	expression tag
Ser -284	-	expression tag
Gly -283	-	expression tag
Arg -282	-	expression tag
Ala -281	-	expression tag
Met -280	-	expression tag
Ala -279	-	expression tag
Ser -278	-	expression tag
Ala -172	Glu 131	engineered mutation

R: 2 - 309 (UniProt: P59540)
R: 310 - 477 (PDB: 7XP4)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	184561.1
All*	Total formula weight	184561.1

*Water molecules are not included.