7XOV

Structural insights into human brain gut peptide cholecystokinin receptors

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44284.1	1	UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	338	37198.7	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody 35	polymer	126	13711.3	1		synthetic construct
5	R	Cholecystokinin receptor type A	polymer	428	47886.1	1	UniProt (P32238) Pfam (PF09193) Pfam (PF00001) In PDB	Homo sapiens (human)	CCK-A receptor,CCK-AR,Cholecystokinin-1 receptor,CCK1-R
6	R	2-[2-[[4-(4-chloranyl-2,5-dimethoxy-phenyl)-5-(2-cyclohexylethyl)-1,3-thiazol-2-yl]carbamoyl]-5,7-dimethyl-indol-1-yl]ethanoic acid	non-polymer		610.2	1	Chemie (IA1)
7		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 394 (UniProt: P63092-2)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 212	engineered mutation
Ala 268	Glu 254	engineered mutation
Lys 271	Asn 257	engineered mutation
Asp 274	Lys 260	engineered mutation
Lys 280	Arg 266	engineered mutation
Asp 284	Thr 270	engineered mutation
Thr 285	Ile 271	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	150941.2
Non-Polymers*	Number of molecules	1
	Total formula weight	610.2
All*	Total formula weight	151551.4

*Water molecules are not included.