7XM0

Crystal structure of Sau3AI-C and DNA substrate complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Type II restriction enzyme Sau3AI	polymer	288	33401.6	3	UniProt (P16667) In PDB	Staphylococcus aureus	R.Sau3AI,Endonuclease Sau3AI,Type-2 restriction enzyme Sau3AI
2	D, E, F, G, H...	DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3')	polymer	10	3044.0	6		synthetic construct
3	A, B, C	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4		water	water		18.0	43	Chemie (HOH)

Sequence modifications

A, B, C: 233 - 489 (UniProt: P16667)

PDB	External Database	Details
Met 212	-	initiating methionine
Gly 213	-	expression tag
Ser 214	-	expression tag
Ser 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
Ser 222	-	expression tag
Ser 223	-	expression tag
Gly 224	-	expression tag
Leu 225	-	expression tag
Val 226	-	expression tag
Pro 227	-	expression tag
Arg 228	-	expression tag
Gly 229	-	expression tag
Ser 230	-	expression tag
His 231	-	expression tag
Met 232	-	expression tag
Cys 490	-	expression tag
Ala 491	-	expression tag
Thr 492	-	expression tag
Gly 493	-	expression tag
Ala 494	-	expression tag
Thr 495	-	expression tag
Cys 496	-	expression tag
Ala 497	-	expression tag
Thr 498	-	expression tag
Gly 499	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	118468.8
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	118541.8

*Water molecules are not included.