7XL4

Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	345	38264.3	2	UniProt (O52760) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Pseudomonas aeruginosa PAO1	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1359	151225.3	1	UniProt (Q51561) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Pseudomonas aeruginosa PAO1	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1412	156038.5	1	UniProt (Q9HWC9) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Pseudomonas aeruginosa PAO1	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	88	9783.9	1	UniProt (Q9HTM1) Pfam (PF01192) In PDB	Pseudomonas aeruginosa PAO1	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	G	Transcriptional factor SutA	polymer	109	11497.4	1	UniProt (Q9HTR9) Pfam (PF20661) In PDB	Pseudomonas aeruginosa PAO1
6	F	RNA polymerase sigma factor RpoS	polymer	338	38606.7	1	UniProt (P45684) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Pseudomonas aeruginosa PAO1	Sigma S,Sigma-38
7	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
8	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A, B: 1 - 333 (UniProt: O52760)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

C: 1 - 1357 (UniProt: Q51561)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag

D: 2 - 1399 (UniProt: Q9HWC9)

PDB	External Database	Details
Met 0	-	initiating methionine
Leu 1	-	expression tag
Gly 1400	-	expression tag
Ser 1401	-	expression tag
Gly 1402	-	expression tag
Ser 1403	-	expression tag
Trp 1404	-	expression tag
Ser 1405	-	expression tag
His 1406	-	expression tag
Pro 1407	-	expression tag
Gln 1408	-	expression tag
Phe 1409	-	expression tag
Glu 1410	-	expression tag
Lys 1411	-	expression tag

G: 1 - 105 (UniProt: Q9HTR9)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag

F: 1 - 334 (UniProt: P45684)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	443680.3
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	443835.4

*Water molecules are not included.