7XKF

Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex at lower state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	NB35	polymer	128	13885.4	1		Camelus bactrianus
5	R	Adhesion G-protein coupled receptor G2	polymer	303	34662.3	1	UniProt (Q8CJ12) Pfam (PF00002) In PDB	Mus musculus (house mouse)	G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6
6	R	3-BETA-HYDROXY-5-ANDROSTEN-17-ONE	non-polymer		288.4	1	Chemie (AND)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: 614 - 891 (UniProt: Q8CJ12)

PDB	External Database	Details
Phe 892	-	expression tag
Trp 893	-	expression tag
Phe 894	-	expression tag
Pro 895	-	expression tag
Glu 896	-	expression tag
Lys 897	-	expression tag
Gly 898	-	expression tag
Ala 899	-	expression tag
Ile 900	-	expression tag
Leu 901	-	expression tag
Thr 902	-	expression tag
Asp 903	-	expression tag
Thr 904	-	expression tag
Ser 905	-	expression tag
Val 906	-	expression tag
Lys 907	-	expression tag
Arg 908	-	expression tag
Asn 909	-	expression tag
Asp 910	-	expression tag
Leu 911	-	expression tag
Ser 912	-	expression tag
Ile 913	-	expression tag
Ile 914	-	expression tag
Ser 915	-	expression tag
Gly 916	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	140095.7
Non-Polymers*	Number of molecules	1
	Total formula weight	288.4
All*	Total formula weight	140384.1

*Water molecules are not included.