7XKD
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39489.2 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | NB35 | polymer | 128 | 13885.4 | 1 | Camelus bactrianus | ||
| 5 | E (R) | Adhesion G-protein coupled receptor G2 | polymer | 303 | 34662.3 | 1 | UniProt (Q8CJ12) Pfam (PF00002) | Homo sapiens (human) | G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6 |
| 6 | F (R) | 3-BETA-HYDROXY-5-ANDROSTEN-17-ONE | non-polymer | 288.4 | 1 | Chemie (AND) | |||
| 7 | G (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: 614 - 891 (UniProt: Q8CJ12)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Phe 892 | - | expression tag |
| Trp 893 | - | expression tag |
| Phe 894 | - | expression tag |
| Pro 895 | - | expression tag |
| Glu 896 | - | expression tag |
| Lys 897 | - | expression tag |
| Gly 898 | - | expression tag |
| Ala 899 | - | expression tag |
| Ile 900 | - | expression tag |
| Leu 901 | - | expression tag |
| Thr 902 | - | expression tag |
| Asp 903 | - | expression tag |
| Thr 904 | - | expression tag |
| Ser 905 | - | expression tag |
| Val 906 | - | expression tag |
| Lys 907 | - | expression tag |
| Arg 908 | - | expression tag |
| Asn 909 | - | expression tag |
| Asp 910 | - | expression tag |
| Leu 911 | - | expression tag |
| Ser 912 | - | expression tag |
| Ile 913 | - | expression tag |
| Ile 914 | - | expression tag |
| Ser 915 | - | expression tag |
| Gly 916 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 140095.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 288.4 | |
| All* | Total formula weight | 140384.1 |
