7XKD
Cryo-EM structure of DHEA-ADGRG2-BT-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39489.2 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | NB35 | polymer | 128 | 13885.4 | 1 | Camelus bactrianus | ||
| 5 | E (R) | Adhesion G-protein coupled receptor G2 | polymer | 303 | 34662.3 | 1 | UniProt (Q8CJ12) Pfam (PF00002) | Homo sapiens (human) | G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6 |
| 6 | F (R) | 3-BETA-HYDROXY-5-ANDROSTEN-17-ONE | non-polymer | 288.4 | 1 | Chemie (AND) PubChem (16401868) PubChem (16759247) PubChem (23968375) PubChem (1780097) PubChem (1780096) PubChem (40467152) PubChem (40467153) PubChem (56828038) PubChem (44135505) PubChem (2817714) PubChem (134506) PubChem (719264) PubChem (908452) PubChem (52231287) PubChem (10541387) PubChem (56729336) PubChem (57256396) PubChem (57284772) PubChem (59742782) PubChem (59746156) PubChem (67990409) PubChem (68237944) PubChem (70954143) PubChem (71773373) PubChem (117072372) PubChem (117808186) PubChem (117809719) PubChem (117852738) PubChem (76) PubChem (5881) PubChem (124338913) PubChem (124399614) PubChem (124426528) PubChem (124896980) PubChem (124904762) PubChem (5318142) PubChem (6432532) PubChem (6603817) PubChem (7048561) PubChem (7092995) PubChem (7200985) PubChem (9814492) PubChem (9860744) PubChem (10613370) PubChem (129317291) PubChem (129606881) PubChem (11889770) PubChem (12358560) PubChem (12358563) PubChem (12358565) PubChem (12358566) PubChem (12358567) PubChem (12358568) PubChem (12358571) PubChem (133556210) PubChem (135033063) PubChem (141834013) PubChem (141888130) PubChem (143972217) PubChem (144612018) PubChem (144878624) PubChem (162900265) PubChem (162900266) PubChem (162900267) PubChem (169544448) | |||
| 7 | G (R) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
R: 614 - 891 (UniProt: Q8CJ12)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -17 | - | initiating methionine |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Phe 892 | - | expression tag |
| Trp 893 | - | expression tag |
| Phe 894 | - | expression tag |
| Pro 895 | - | expression tag |
| Glu 896 | - | expression tag |
| Lys 897 | - | expression tag |
| Gly 898 | - | expression tag |
| Ala 899 | - | expression tag |
| Ile 900 | - | expression tag |
| Leu 901 | - | expression tag |
| Thr 902 | - | expression tag |
| Asp 903 | - | expression tag |
| Thr 904 | - | expression tag |
| Ser 905 | - | expression tag |
| Val 906 | - | expression tag |
| Lys 907 | - | expression tag |
| Arg 908 | - | expression tag |
| Asn 909 | - | expression tag |
| Asp 910 | - | expression tag |
| Leu 911 | - | expression tag |
| Ser 912 | - | expression tag |
| Ile 913 | - | expression tag |
| Ile 914 | - | expression tag |
| Ser 915 | - | expression tag |
| Gly 916 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 140095.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 288.4 | |
| All* | Total formula weight | 140384.1 |
