7XK8

Cryo-EM structure of the Neuromedin U receptor 2 (NMUR2) in complex with G Protein and its endogeneous Peptide-Agonist NMU25

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (P)	Neuromedin-U-25	polymer	26	3083.4	1	UniProt (P48645) Pfam (PF02070)	Homo sapiens	NmU-25
2	B (R)	Neuromedin-U receptor 2	polymer	393	44702.0	1	UniProt (Q9GZQ4) Pfam (PF00001)	Homo sapiens (human)	NMU-R2,G-protein coupled receptor FM-4,G-protein coupled receptor TGR-1
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I

Sequence modifications

P: 1 - 25 (UniProt: P48645)

PDB	External Database	Details
Nh2 26	-	amidation

R: 1 - 345 (UniProt: Q9GZQ4)

PDB	External Database	Details
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Pro 0	-	expression tag
Glu 346	-	expression tag
Phe 347	-	expression tag
Leu 348	-	expression tag
Glu 349	-	expression tag
Val 350	-	expression tag
Leu 351	-	expression tag
Phe 352	-	expression tag
Gln 353	-	expression tag
Gly 354	-	expression tag
Pro 355	-	expression tag
Trp 356	-	expression tag
Ser 357	-	expression tag
His 358	-	expression tag
Pro 359	-	expression tag
Gln 360	-	expression tag
Phe 361	-	expression tag
Glu 362	-	expression tag
Lys 363	-	expression tag
Gly 364	-	expression tag
Gly 365	-	expression tag
Gly 366	-	expression tag
Ser 367	-	expression tag
Gly 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Ser 371	-	expression tag
Gly 372	-	expression tag
Gly 373	-	expression tag
Ser 374	-	expression tag
Ala 375	-	expression tag
Trp 376	-	expression tag
Ser 377	-	expression tag
His 378	-	expression tag
Pro 379	-	expression tag
Gln 380	-	expression tag
Phe 381	-	expression tag
Glu 382	-	expression tag
Lys 383	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	134838.0
All*	Total formula weight	134838.0

*Water molecules are not included.