7XJK

Cryo-EM structure of the galanin-bound GALR2-miniGq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Galanin	polymer	30	3161.4	1	UniProt (P22466) Pfam (PF01296)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(q)	polymer	246	28026.8	1		Homo sapiens
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	348	38389.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (E)	single Fab chain (svFv16)	polymer	300	32071.6	1		Homo sapiens
6	F (F)	Galanin receptor type 2	polymer	332	36401.9	1	UniProt (O43603) Pfam (PF00001)	Homo sapiens (human)	GAL2-R,GALR-2

Sequence modifications

C: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

F: 1 - 314 (UniProt: O43603)

PDB	External Database	Details
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gln 165	Arg 165	conflict
Gly 315	-	expression tag
Ser 316	-	expression tag
Leu 317	-	expression tag
Glu 318	-	expression tag
Val 319	-	expression tag
Leu 320	-	expression tag
Phe 321	-	expression tag
Gln 322	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	145896.7
All*	Total formula weight	145896.7

*Water molecules are not included.