7XJJ
Cryo-EM structure of the galanin-bound GALR1-miniGo complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | G protein subunit alpha o1,Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 228 | 25447.1 | 1 | UniProt (A0A1W2PRJ7) UniProt (A0A1W2PP38) UniProt (P09471) Pfam (PF00503) In PDB | Homo sapiens (human) | |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | C | Galanin | polymer | 30 | 3161.4 | 1 | UniProt (P22466) Pfam (PF01296) In PDB | Homo sapiens | |
4 | E | Galanin receptor type 1 | polymer | 347 | 38382.9 | 1 | UniProt (P47211) Pfam (PF00001) In PDB | Homo sapiens (human) | GAL1-R,GALR-1 |
5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
6 | S | single Fab chain (svFv16) | polymer | 298 | 31857.4 | 1 | Homo sapiens |
Sequence modifications
A: 1 - 173 (UniProt: A0A1W2PRJ7)
A: 182 - 231 (UniProt: A0A1W2PP38)
A: 242 - 354 (UniProt: P09471)
E: 1 - 322 (UniProt: P47211)
G: 1 - 71 (UniProt: P59768)
PDB | External Database | Details |
---|---|---|
Asp 42 | Gly 42 | conflict |
Asn 43 | Glu 43 | conflict |
Gly 174 | - | linker |
Gly 175 | - | linker |
Ser 176 | - | linker |
Gly 177 | - | linker |
Gly 178 | - | linker |
Ser 179 | - | linker |
Gly 180 | - | linker |
Gly 181 | - | linker |
PDB | External Database | Details |
---|---|---|
Asp 227 | Ala 69 | conflict |
Asp 230 | Gly 72 | conflict |
PDB | External Database | Details |
---|---|---|
Ala 332 | Ile 332 | conflict |
Ile 335 | Val 335 | conflict |
PDB | External Database | Details |
---|---|---|
Asp -9 | - | expression tag |
Tyr -8 | - | expression tag |
Lys -7 | - | expression tag |
Asp -6 | - | expression tag |
Asp -5 | - | expression tag |
Asp -4 | - | expression tag |
Asp -3 | - | expression tag |
Lys -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 323 | - | expression tag |
Gly 324 | - | expression tag |
Gly 325 | - | expression tag |
Gly 326 | - | expression tag |
Gly 327 | - | expression tag |
Gly 328 | - | expression tag |
Ala 329 | - | expression tag |
Gly 330 | - | expression tag |
Ala 331 | - | expression tag |
Leu 332 | - | expression tag |
Glu 333 | - | expression tag |
Val 334 | - | expression tag |
Leu 335 | - | expression tag |
Phe 336 | - | expression tag |
Gln 337 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 144110.9 | |
All* | Total formula weight | 144110.9 |