7XJG

Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.5 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RNA-directed DNA polymerase from retron EC86	polymer	330	37832.1	2	UniProt (P23070) Pfam (PF00078) In PDB	Escherichia coli	EC86-RT,Reverse transcriptase
2	C, J	retron St85 family effector protein	polymer	307	35538.2	2	UniProt (A0A140NAX8) UniProt (by SIFTS) (P0DV88) In PDB	Escherichia coli
3	D, G	DNA (105-MER)	polymer	105	32468.8	2	GenBank (341167)	Escherichia coli
4	E, H	RNA (81-MER)	polymer	81	25870.2	2	GenBank (341167)	Escherichia coli
5	F, I	RNA (14-MER)	polymer	14	4541.8	2		Escherichia coli
6	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 320 (UniProt: P23070)

PDB	External Database	Details
Leu 321	-	expression tag
Glu 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	272502.2
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	272550.8

*Water molecules are not included.