7XIH

Crystal structure of the aminopropyltransferase, SpeE from hyperthermophilic crenarchaeon, Pyrobaculum calidifontis in complex with 5'-methylthioadenosine (MTA) and spermidine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Polyamine aminopropyltransferase	polymer	309	34859.8	2	UniProt (A3MU81)	Pyrobaculum calidifontis JCM 11548	Putrescine aminopropyltransferase,PAPT,Spermidine synthase,SPDS,SPDSY
2	C, E (A, B)	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	2	Chemie (MTA)
3	D, F (A, B)	SPERMIDINE	non-polymer		145.2	2	Chemie (SPD)
4	G, H (A, B)	water	water		18.0	554	Chemie (HOH)

Sequence modifications

A, B: 1 - 289 (UniProt: A3MU81)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69719.5
Non-Polymers*	Number of molecules	4
	Total formula weight	885.2
All*	Total formula weight	70604.7

*Water molecules are not included.