7XBN
Crystal Structure of YC-17-bound cytochrome P450 PikC with the unnatural amino acid p-Acetyl-L-Phenylalanine incorporated at position 238
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Cytochrome P450 monooxygenase PikC | polymer | 444 | 49390.9 | 2 | UniProt (O87605) Pfam (PF00067) In PDB | Streptomyces venezuelae | Cytochrome P450 monooxygenase PicK,Narbomycin C-12 hydroxylase,Pikromycin synthase CYP107L1 |
2 | A, B | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
3 | A, B | 4-{[4-(DIMETHYLAMINO)-3-HYDROXY-6-METHYLTETRAHYDRO-2H-PYRAN-2-YL]OXY}-12-ETHYL-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE | non-polymer | 453.6 | 2 | Chemie (PXI) | |||
4 | A, B | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
5 | A, B | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
6 | water | water | 18.0 | 877 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 416 (UniProt: O87605)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
4af 238 | His 238 | conflict |
Leu 417 | - | expression tag |
Glu 418 | - | expression tag |
His 419 | - | expression tag |
His 420 | - | expression tag |
His 421 | - | expression tag |
His 422 | - | expression tag |
His 423 | - | expression tag |
His 424 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 98781.9 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 2826.9 | |
All* | Total formula weight | 101608.8 |