7XB5

Structure of the ligand-binding domain of S. cerevisiae Upc2 in fusion with T4 lysozyme

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	fusion protein of Sterol uptake control protein 2 and Endolysin	polymer	465	52253.8	1	UniProt (Q12151) UniProt (P00720) Pfam (PF11951) Pfam (PF00959)	Saccharomyces cerevisiae S288C	Mannoprotein regulation by oxygen protein 4,Lysis protein,Lysozyme,Muramidase

Sequence modifications

A: 602 - 714 (UniProt: Q12151)

PDB	External Database	Details
Met 581	-	initiating methionine
Gly 582	-	expression tag
Ser 583	-	expression tag
Ser 584	-	expression tag
His 585	-	expression tag
His 586	-	expression tag
His 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag
His 590	-	expression tag
Ser 591	-	expression tag
Ser 592	-	expression tag
Gly 593	-	expression tag
Leu 594	-	expression tag
Val 595	-	expression tag
Pro 596	-	expression tag
Arg 597	-	expression tag
Gly 598	-	expression tag
Ser 599	-	expression tag
His 600	-	expression tag
Met 601	-	expression tag

A: 717 - 876 (UniProt: P00720)

PDB	External Database	Details
Gly 727	Arg 12	engineered mutation
Thr 769	Cys 54	engineered mutation
Ala 812	Cys 97	engineered mutation
Arg 852	Ile 137	engineered mutation
Val 877	-	linker
Asp 878	-	linker

A: 879 - 1045 (UniProt: Q12151)

PDB	External Database	Details
Val 715	-	linker
Asp 716	-	linker
-	Pro 772	deletion
-	Asp 773	deletion
-	Val 774	deletion
-	Gly 775	deletion
-	Thr 776	deletion
Gly 925	Ile 777	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52253.8
All*	Total formula weight	52253.8

*Water molecules are not included.