7X9C

Cryo-EM structure of neuropeptide Y Y4 receptor in complex with PP and Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Neuropeptide Y receptor type 4	polymer	380	42755.2	1	UniProt (P50391) Pfam (PF00001)	Homo sapiens (human)	NPY4-R,Pancreatic polypeptide receptor 1,PP1
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (P)	Pancreatic hormone	polymer	36	4185.7	1	UniProt (P01298) Pfam (PF00159)	Homo sapiens	PH

Sequence modifications

R: 1 - 342 (UniProt: P50391)

PDB	External Database	Details
Glu 343	-	expression tag
Phe 344	-	expression tag
Leu 345	-	expression tag
Glu 346	-	expression tag
Val 347	-	expression tag
Leu 348	-	expression tag
Phe 349	-	expression tag
Gln 350	-	expression tag
Gly 351	-	expression tag
Pro 352	-	expression tag
Trp 353	-	expression tag
Ser 354	-	expression tag
His 355	-	expression tag
Pro 356	-	expression tag
Gln 357	-	expression tag
Phe 358	-	expression tag
Glu 359	-	expression tag
Lys 360	-	expression tag
Gly 361	-	expression tag
Gly 362	-	expression tag
Gly 363	-	expression tag
Ser 364	-	expression tag
Gly 365	-	expression tag
Gly 366	-	expression tag
Gly 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Ser 371	-	expression tag
Ala 372	-	expression tag
Trp 373	-	expression tag
Ser 374	-	expression tag
His 375	-	expression tag
Pro 376	-	expression tag
Gln 377	-	expression tag
Phe 378	-	expression tag
Glu 379	-	expression tag
Lys 380	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	133993.6
All*	Total formula weight	133993.6

*Water molecules are not included.