7X9A

Cryo-EM structure of neuropeptide Y Y1 receptor in complex with NPY and Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Neuropeptide Y receptor type 1	polymer	378	43462.6	1	UniProt (P25929) Pfam (PF00001) In PDB	Homo sapiens (human)	NPY1-R
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	Pfam (PF00631) UniProt (by SIFTS) (P59768) In PDB	Homo sapiens
5	P	Neuropeptide Y	polymer	36	4277.7	1	UniProt (P01303) Pfam (PF00159) In PDB	Homo sapiens	Neuropeptide tyrosine,NPY

Sequence modifications

R: 1 - 340 (UniProt: P25929)

PDB	External Database	Details
Glu 341	-	expression tag
Phe 342	-	expression tag
Leu 343	-	expression tag
Glu 344	-	expression tag
Val 345	-	expression tag
Leu 346	-	expression tag
Phe 347	-	expression tag
Gln 348	-	expression tag
Gly 349	-	expression tag
Pro 350	-	expression tag
Trp 351	-	expression tag
Ser 352	-	expression tag
His 353	-	expression tag
Pro 354	-	expression tag
Gln 355	-	expression tag
Phe 356	-	expression tag
Glu 357	-	expression tag
Lys 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Gly 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Gly 364	-	expression tag
Gly 365	-	expression tag
Ser 366	-	expression tag
Gly 367	-	expression tag
Gly 368	-	expression tag
Ser 369	-	expression tag
Ala 370	-	expression tag
Trp 371	-	expression tag
Ser 372	-	expression tag
His 373	-	expression tag
Pro 374	-	expression tag
Gln 375	-	expression tag
Phe 376	-	expression tag
Glu 377	-	expression tag
Lys 378	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	134759.9
All*	Total formula weight	134759.9

*Water molecules are not included.