7X74

Cryo-EM structure of Streptomyces coelicolor transcription initial complex with two Zur dimers.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	340	36734.6	2	UniProt (A0A6G2M9E1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Streptomyces coelicolor A3(2)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1161	128644.9	1	UniProt (Q9L0L0) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Streptomyces coelicolor A3(2)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1307	145912.2	1	UniProt (Q8CJT1) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Streptomyces coelicolor A3(2)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	90	9716.9	1	UniProt (Q9KXS1) Pfam (PF01192) In PDB	Streptomyces coelicolor A3(2)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase principal sigma factor HrdB	polymer	531	58189.0	1	UniProt (P18183) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Streptomyces coelicolor A3(2)	RNA polymerase sigma factor SigA
6	G, H, M, N	Putative metal uptake regulation protein	polymer	159	16904.8	4	UniProt (Q9L2H5) Pfam (PF01475) In PDB	Streptomyces coelicolor A3(2)	Zur
7	O	DNA (84-MER)	polymer	84	25808.4	1	GenBank (24418971)	Streptomyces coelicolor A3(2)
8	P	DNA (84-MER)	polymer	84	26008.5	1		Streptomyces coelicolor A3(2)
9	Q	RNA	polymer	5	1626.0	1		Streptomyces coelicolor A3(2)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	N, D, G, H, M	ZINC ION	non-polymer		65.4	14	Chemie (ZN)

Sequence modifications

D: 1 - 1299 (UniProt: Q8CJT1)

PDB	External Database	Details
His 1300	-	expression tag
His 1301	-	expression tag
His 1302	-	expression tag
His 1303	-	expression tag
His 1304	-	expression tag
His 1305	-	expression tag
His 1306	-	expression tag
His 1307	-	expression tag

F: 1 - 511 (UniProt: P18183)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

G, H, M, N: 1 - 139 (UniProt: Q9L2H5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	536994.7
Non-Polymers*	Number of molecules	15
	Total formula weight	940.0
All*	Total formula weight	537934.7

*Water molecules are not included.