7X6I

Cryo-EM structure of the human TRPC5 ion channel in complex with G alpha i3 subunits, class1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E (A, B, C, D)	Short transient receptor potential channel 5	polymer	773	89951.9	4	UniProt (Q9UL62) Pfam (PF12796) Pfam (PF00023) Pfam (PF08344) Pfam (PF00520)	Homo sapiens (human)	TrpC5,Transient receptor protein 5,TRP-5,hTRP-5,hTRP5
2	B, F, G, H (E, F, G, H)	Guanine nucleotide-binding protein G(i) subunit alpha-3	polymer	360	41399.0	4	UniProt (P08754) Pfam (PF00503)	Homo sapiens (human)	G(i) alpha-3
3	I, P, U, Z (A, B, C, D)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	4	Chemie (PTY)
4	AA, J, Q, V (D, A, B, C)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	4	Chemie (Y01)
5	BA, K, R, W (D, A, B, C)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
6	CA, L, S, X (D, A, B, C)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
7	M, N, T, Y (A, B, C)	(2S)-2-(hexadecanoyloxy)-3-hydroxypropyl (9Z)-octadec-9-enoate	non-polymer		594.9	4	Chemie (YZY)
8	DA, EA, FA, O (F, G, H, E)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	4	Chemie (GTP)

Sequence modifications

A, B, C, D: 1 - 765 (UniProt: Q9UL62)

PDB	External Database	Details
Ser 766	-	expression tag
Arg 767	-	expression tag
Leu 768	-	expression tag
Glu 769	-	expression tag
Val 770	-	expression tag
Leu 771	-	expression tag
Phe 772	-	expression tag
Gln 773	-	expression tag

E, F, G, H: 1 - 110 (UniProt: P08754)
E, F, G, H: 110 - 354 (UniProt: P08754)

PDB	External Database	Details
His 110	-	linker
His 110	-	linker
His 110	-	linker
His 110	-	linker
His 110	-	linker
His 110	-	linker
Leu 204	Gln 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	525403.8
Non-Polymers*	Number of molecules	24
	Total formula weight	9777.5
All*	Total formula weight	535181.3

*Water molecules are not included.