7X5H

Serotonin 5A (5-HT5A) receptor-Gi protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (E)	scFv16	polymer	286	30552.2	1		Mus musculus
5	E (R)	Soluble cytochrome b562,5-hydroxytryptamine receptor 5A,hydroxytryptamine receptor 5A	polymer	690	77127.3	1	UniProt (P0ABE7) UniProt (P47898) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,5-HT-5,5-HT-5A,5-HT5A,Serotonin receptor 5A
6	F (R)	3-(2-azanylethyl)-1H-indole-5-carboxamide	non-polymer		203.2	1	Chemie (8K3)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Met -159	-	initiating methionine
Lys -158	-	expression tag
Thr -157	-	expression tag
Ile -156	-	expression tag
Ile -155	-	expression tag
Ala -154	-	expression tag
Leu -153	-	expression tag
Ser -152	-	expression tag
Tyr -151	-	expression tag
Ile -150	-	expression tag
Phe -149	-	expression tag
Cys -148	-	expression tag
Leu -147	-	expression tag
Val -146	-	expression tag
Phe -145	-	expression tag
Ala -144	-	expression tag
Asp -143	-	expression tag
Tyr -142	-	expression tag
Lys -141	-	expression tag
Asp -140	-	expression tag
Asp -139	-	expression tag
Asp -138	-	expression tag
Asp -137	-	expression tag
Ala -136	-	expression tag
Lys -135	-	expression tag
Leu -134	-	expression tag
Gln -133	-	expression tag
Thr -132	-	expression tag
Met -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Ala -9	-	linker
Ser -8	-	linker
Glu -7	-	linker
Asn -6	-	linker
Leu -5	-	linker
Tyr -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Gly 0	-	linker
Thr 1	-	linker

R: 2 - 357 (UniProt: P47898)
R: 358 - 530 (PDB: 7X5H)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	196050.5
Non-Polymers*	Number of molecules	1
	Total formula weight	203.2
All*	Total formula weight	196253.7

*Water molecules are not included.