7X2F

Cryo-EM structure of the dopamine and LY3154207-bound D1 dopamine receptor and mini-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (F)	D(1A) dopamine receptor	polymer	473	52409.7	1	UniProt (P21728) Pfam (PF00001)	Homo sapiens (human)	Dopamine D1 receptor
2	B (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	28907.7	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens
3	C (E)	Nanobody35	polymer	160	17352.5	1		synthetic construct
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F, H (F)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
7	G (F)	2-[2,6-bis(chloranyl)phenyl]-1-[(1S,3R)-3-(hydroxymethyl)-1-methyl-5-(3-methyl-3-oxidanyl-butyl)-3,4-dihydro-1H-isoquinolin-2-yl]ethanone	non-polymer		450.4	1	Chemie (G4C)
8	I (F)	L-DOPAMINE	non-polymer		153.2	1	Chemie (LDP)

Sequence modifications

F: 1 - 446 (UniProt: P21728)

PDB	External Database	Details
Met -26	-	initiating methionine
Lys -25	-	expression tag
Thr -24	-	expression tag
Ile -23	-	expression tag
Ile -22	-	expression tag
Ala -21	-	expression tag
Leu -20	-	expression tag
Ser -19	-	expression tag
Tyr -18	-	expression tag
Ile -17	-	expression tag
Phe -16	-	expression tag
Cys -15	-	expression tag
Leu -14	-	expression tag
Val -13	-	expression tag
Phe -12	-	expression tag
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Ile -1	-	expression tag
Asp 0	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	145933.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1376.9
All*	Total formula weight	147309.9

*Water molecules are not included.