7X24
Cryo-EM structure of non gastric H,K-ATPase alpha2 SPWC mutant in (2K+)E2-AlF state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Potassium-transporting ATPase alpha chain 2 | polymer | 986 | 109145.7 | 1 | UniProt (P54708) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF00702) Pfam (PF00689) | Rattus norvegicus | HK alpha 2,Non-gastric H(+)/K(+) ATPase subunit alpha,Non-gastric Na(+)/K(+) ATPase subunit alpha,Proton pump,Sodium pump |
| 2 | B (B) | Sodium/potassium-transporting ATPase subunit beta-1 | polymer | 324 | 37516.9 | 1 | UniProt (P07340) Pfam (PF00287) | Rattus norvegicus | Sodium/potassium-dependent ATPase subunit beta-1 |
| 3 | C, D, E (A) | POTASSIUM ION | non-polymer | 39.1 | 3 | Chemie (K) | |||
| 4 | F, H, J, K, L (A) | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 5 | Chemie (PCW) | |||
| 5 | G, I, P (A, B) | CHOLESTEROL | non-polymer | 386.7 | 3 | Chemie (CLR) | |||
| 6 | M (A) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) | |||
| 7 | N (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | O (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) |
Sequence modifications
A: 53 - 1036 (UniProt: P54708)
B: 1 - 304 (UniProt: P07340)
| PDB | External Database | Details |
|---|---|---|
| Gly 51 | - | expression tag |
| Met 52 | - | expression tag |
| Ser 794 | Lys 794 | engineered mutation |
| Pro 797 | Ala 797 | engineered mutation |
| Trp 943 | Ile 943 | engineered mutation |
| Cys 949 | Arg 949 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Asp -17 | - | expression tag |
| Tyr -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Lys -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 146662.5 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 5561.3 | |
| All* | Total formula weight | 152223.9 |
