7X20

Crystal structure of non gastric H,K-ATPase alpha2 in (K+)E2-AlF state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Potassium-transporting ATPase alpha chain 2	polymer	986	109084.7	1	UniProt (P54708) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF00702) Pfam (PF00689)	Rattus norvegicus	HK alpha 2,Non-gastric H(+)/K(+) ATPase subunit alpha,Non-gastric Na(+)/K(+) ATPase subunit alpha,Proton pump,Sodium pump
2	B (B)	Sodium/potassium-transporting ATPase subunit beta-1	polymer	324	37516.9	1	UniProt (P07340) Pfam (PF00287)	Rattus norvegicus	Sodium/potassium-dependent ATPase subunit beta-1
3	C (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
4	D, E (A)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	F (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	G (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 53 - 1036 (UniProt: P54708)

PDB	External Database	Details
Gly 51	-	expression tag
Met 52	-	expression tag
Gly 315	Asp 315	conflict

B: 1 - 304 (UniProt: P07340)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	146601.5
Non-Polymers*	Number of molecules	4
	Total formula weight	402.4
All*	Total formula weight	147003.9

*Water molecules are not included.