7WYV

Cryo-EM structure of Na+,K+-ATPase in the E2P state formed by ATP in the presence of 40 mM Mg2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, C)	Sodium/potassium-transporting ATPase subunit alpha	polymer	1028	113389.9	2	UniProt (Q4H132) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF00702) Pfam (PF00689)	Squalus acanthias (spiny dogfish)
2	B, E (B, D)	Na+,K+-ATPase beta subunit	polymer	305	35176.1	2	UniProt (C4IX13) Pfam (PF00287)	Squalus acanthias (spiny dogfish)	Sodium/potassium-transporting ATPase subunit beta-1
3	C, F (G, E)	FXYD domain-containing ion transport regulator	polymer	94	10195.8	2	UniProt (Q70Q12) Pfam (PF02038)	Squalus acanthias (spiny dogfish)
4	G, K (F, K)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1057.0	2	In PDB GlyTouCan (G82348BZ)
5	H, L (H, L)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1114.0	2	In PDB GlyTouCan (G61751GZ)
6	I, M (I, M)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	2	In PDB GlyTouCan (G42466VF)
7	J, N (J, N)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
8	IA, KA, O, Q (C, A)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
9	JA, P (C, A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
10	LA, R (C, A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
11	EA, FA, MA, NA, S... (A, B, C, D)	CHOLESTEROL	non-polymer		386.7	8	Chemie (CLR)
12	AA, AB, BA, BB, CA... (A, E, G, C)	1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		787.1	24	Chemie (PCW)
13	CB, DB (A, C)	water	water		18.0	10	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	317523.7
Branched	Number of molecules	8
	Total formula weight	6980.4
Non-Polymers*	Number of molecules	40
	Total formula weight	23141.7
All*	Total formula weight	347645.8

*Water molecules are not included.