7WVU

Cryo-EM structure of the human formyl peptide receptor 1 in complex with fMLF and Gi1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	fMet-Leu-Phe receptor	polymer	371	40754.7	1	UniProt (P21462) Pfam (PF00001)	Homo sapiens (human)	fMLP receptor,N-formyl peptide receptor,FPR,N-formylpeptide chemoattractant receptor
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (L)	FME-LEU-PHE	polymer	3	437.6	1		Homo sapiens

Sequence modifications

R: 2 - 321 (UniProt: P21462)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Glu 322	-	expression tag
Phe 323	-	expression tag
Leu 324	-	expression tag
Glu 325	-	expression tag
Val 326	-	expression tag
Leu 327	-	expression tag
Phe 328	-	expression tag
Gln 329	-	expression tag
Gly 330	-	expression tag
Pro 331	-	expression tag
Gly 332	-	expression tag
Ser 333	-	expression tag
Trp 334	-	expression tag
Ser 335	-	expression tag
His 336	-	expression tag
Pro 337	-	expression tag
Gln 338	-	expression tag
Phe 339	-	expression tag
Glu 340	-	expression tag
Lys 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Ala 345	-	expression tag
Gly 346	-	expression tag
Ala 347	-	expression tag
Ser 348	-	expression tag
Ala 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Trp 352	-	expression tag
Ser 353	-	expression tag
His 354	-	expression tag
Pro 355	-	expression tag
Gln 356	-	expression tag
Phe 357	-	expression tag
Glu 358	-	expression tag
Lys 359	-	expression tag
Gly 360	-	expression tag
Ser 361	-	expression tag
Asp 362	-	expression tag
Tyr 363	-	expression tag
Lys 364	-	expression tag
Asp 365	-	expression tag
Asp 366	-	expression tag
Asp 367	-	expression tag
Asp 368	-	expression tag
Lys 369	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	128244.9
All*	Total formula weight	128244.9

*Water molecules are not included.