7WUJ

Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	mini-Gs	polymer	361	41879.5	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) In PDB	Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	E	Adhesion G-protein coupled receptor G4,Uncharacterized protein	polymer	631	71628.0	1	UniProt (Q8IZF6) UniProt (A0A366VY15) Pfam (PF00002) In PDB	Homo sapiens (human)	G-protein coupled receptor 112
4	S	scFv16	polymer	250	26610.6	1		synthetic construct
5	Y	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
6	N	Nanobody-35	polymer	128	13885.4	1		Lama glama

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

E: 2720 - 3080 (UniProt: Q8IZF6)

PDB	External Database	Details
Met 2704	-	expression tag
Lys 2705	-	expression tag
Thr 2706	-	expression tag
Ile 2707	-	expression tag
Ile 2708	-	expression tag
Ala 2709	-	expression tag
Leu 2710	-	expression tag
Ser 2711	-	expression tag
Tyr 2712	-	expression tag
Ile 2713	-	expression tag
Phe 2714	-	expression tag
Cys 2715	-	expression tag
Leu 2716	-	expression tag
Val 2717	-	expression tag
Phe 2718	-	expression tag
Ala 2719	-	expression tag
His 3081	-	linker
His 3082	-	linker
His 3083	-	linker
His 3084	-	linker
His 3085	-	linker
His 3086	-	linker
His 3087	-	linker
His 3088	-	linker
Gly 3089	-	linker
Ser 3090	-	linker
Ala 3091	-	linker
Glu 3092	-	linker
Asn 3093	-	linker
Leu 3094	-	linker
Tyr 3095	-	linker
Phe 3096	-	linker
Gln 3097	-	linker
Gly 3098	-	linker

E: 3099 - 3334 (UniProt: A0A366VY15)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	201353.8
All*	Total formula weight	201353.8

*Water molecules are not included.