7WUI

Tethered peptide activation mechanism of adhesion GPCRs ADGRG2 and ADGRG4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	mini-Gs	polymer	361	41879.5	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) In PDB	Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody-35	polymer	128	13885.4	1		Lama glama
5	R	Adhesion G-protein coupled receptor G2,mCherry	polymer	1149	126852.2	1	UniProt (Q8CJ12) Pfam (PF01825) Pfam (PF00002) In PDB	Mus musculus (house mouse)	G-protein coupled receptor 64,Mouse epididymis-specific protein 6,Me6
6	S	scFv16	polymer	250	26610.6	1		synthetic construct
7	L	IP15	polymer	15	1646.9	1	UniProt (by SIFTS) (Q8CJ12) In PDB	synthetic construct

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: 38 - 891 (UniProt: Q8CJ12)

PDB	External Database	Details
Met 22	-	expression tag
Lys 23	-	expression tag
Thr 24	-	expression tag
Ile 25	-	expression tag
Ile 26	-	expression tag
Ala 27	-	expression tag
Leu 28	-	expression tag
Ser 29	-	expression tag
Tyr 30	-	expression tag
Ile 31	-	expression tag
Phe 32	-	expression tag
Cys 33	-	expression tag
Leu 34	-	expression tag
Val 35	-	expression tag
Phe 36	-	expression tag
Ala 37	-	expression tag
Ala 597	His 597	engineered mutation
Ala 599	Thr 599	engineered mutation
Phe 892	-	linker
Trp 893	-	linker
Phe 894	-	linker
Pro 895	-	linker
Glu 896	-	linker
Lys 897	-	linker
Gly 898	-	linker
Ala 899	-	linker
Ile 900	-	linker
Leu 901	-	linker
Thr 902	-	linker
Asp 903	-	linker
Thr 904	-	linker
Ser 905	-	linker
Val 906	-	linker
Lys 907	-	linker
Arg 908	-	linker
Asn 909	-	linker
Asp 910	-	linker
Leu 911	-	linker
Ser 912	-	linker
Ile 913	-	linker
Ile 914	-	linker
Ser 915	-	linker
Gly 916	-	linker
His 917	-	linker
His 918	-	linker
His 919	-	linker
His 920	-	linker
His 921	-	linker
His 922	-	linker
His 923	-	linker
His 924	-	linker
Gly 925	-	linker
Ser 926	-	linker
Ala 927	-	linker
Glu 928	-	linker
Asn 929	-	linker
Leu 930	-	linker
Tyr 931	-	linker
Phe 932	-	linker
Gln 933	-	linker
Gly 934	-	linker

R: 935 - 1170 (PDB: 7WUI)

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	256739.2
All*	Total formula weight	256739.2

*Water molecules are not included.