7WU3

Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	243	28387.1	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nanobody Nb35	polymer	162	17540.7	1		Lama glama
5	R	Adhesion G-protein coupled receptor F1	polymer	674	74610.5	1	UniProt (Q5T601) Pfam (PF01825) Pfam (PF00002) In PDB	Homo sapiens (human)	G protein-coupled receptor 110,G protein-coupled receptor KPG_012,G protein-coupled receptor PGR19
6	R	[(2~{R})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate	non-polymer		496.6	1	Chemie (K6G)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 251 - 860 (UniProt: Q5T601)

PDB	External Database	Details
Met 225	-	initiating methionine
Lys 226	-	expression tag
Thr 227	-	expression tag
Ile 228	-	expression tag
Ile 229	-	expression tag
Ala 230	-	expression tag
Leu 231	-	expression tag
Ser 232	-	expression tag
Tyr 233	-	expression tag
Ile 234	-	expression tag
Phe 235	-	expression tag
Cys 236	-	expression tag
Leu 237	-	expression tag
Val 238	-	expression tag
Phe 239	-	expression tag
Ala 240	-	expression tag
Asp 241	-	expression tag
Tyr 242	-	expression tag
Lys 243	-	expression tag
Asp 244	-	expression tag
Asp 245	-	expression tag
Asp 246	-	expression tag
Asp 247	-	expression tag
Gly 248	-	expression tag
Ala 249	-	expression tag
Pro 250	-	expression tag
Glu 861	-	expression tag
Phe 862	-	expression tag
Leu 863	-	expression tag
Glu 864	-	expression tag
Val 865	-	expression tag
Leu 866	-	expression tag
Phe 867	-	expression tag
Gln 868	-	expression tag
Gly 869	-	expression tag
Pro 870	-	expression tag
Trp 871	-	expression tag
Ser 872	-	expression tag
His 873	-	expression tag
Pro 874	-	expression tag
Gln 875	-	expression tag
Phe 876	-	expression tag
Glu 877	-	expression tag
Lys 878	-	expression tag
Gly 879	-	expression tag
Gly 880	-	expression tag
Gly 881	-	expression tag
Ser 882	-	expression tag
Gly 883	-	expression tag
Gly 884	-	expression tag
Gly 885	-	expression tag
Ser 886	-	expression tag
Gly 887	-	expression tag
Gly 888	-	expression tag
Ser 889	-	expression tag
Ala 890	-	expression tag
Trp 891	-	expression tag
Ser 892	-	expression tag
His 893	-	expression tag
Pro 894	-	expression tag
Gln 895	-	expression tag
Phe 896	-	expression tag
Glu 897	-	expression tag
Lys 898	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	167143.9
Non-Polymers*	Number of molecules	1
	Total formula weight	496.6
All*	Total formula weight	167640.5

*Water molecules are not included.