7WU2

Cryo-EM structure of the adhesion GPCR ADGRD1 in complex with miniGs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	243	28387.1	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens	G gamma-I
4	N	Nanobody Nb35	polymer	162	17540.7	1		Lama glama
5	R	Adhesion G-protein coupled receptor D1	polymer	348	38168.6	1	UniProt (Q6QNK2) Pfam (PF00002) In PDB	Homo sapiens (human)	G-protein coupled receptor 133,G-protein coupled receptor PGR25

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 545 - 827 (UniProt: Q6QNK2)

PDB	External Database	Details
Met 526	-	initiating methionine
Lys 527	-	expression tag
Thr 528	-	expression tag
Ile 529	-	expression tag
Ile 530	-	expression tag
Ala 531	-	expression tag
Leu 532	-	expression tag
Ser 533	-	expression tag
Tyr 534	-	expression tag
Ile 535	-	expression tag
Phe 536	-	expression tag
Cys 537	-	expression tag
Leu 538	-	expression tag
Val 539	-	expression tag
Phe 540	-	expression tag
Ala 541	-	expression tag
Gly 542	-	expression tag
Ala 543	-	expression tag
Pro 544	-	expression tag
Glu 828	-	expression tag
Phe 829	-	expression tag
Leu 830	-	expression tag
Glu 831	-	expression tag
Val 832	-	expression tag
Leu 833	-	expression tag
Phe 834	-	expression tag
Gln 835	-	expression tag
Gly 836	-	expression tag
Pro 837	-	expression tag
Trp 838	-	expression tag
Ser 839	-	expression tag
His 840	-	expression tag
Pro 841	-	expression tag
Gln 842	-	expression tag
Phe 843	-	expression tag
Glu 844	-	expression tag
Lys 845	-	expression tag
Gly 846	-	expression tag
Gly 847	-	expression tag
Gly 848	-	expression tag
Ser 849	-	expression tag
Gly 850	-	expression tag
Gly 851	-	expression tag
Gly 852	-	expression tag
Ser 853	-	expression tag
Gly 854	-	expression tag
Gly 855	-	expression tag
Ser 856	-	expression tag
Ala 857	-	expression tag
Trp 858	-	expression tag
Ser 859	-	expression tag
His 860	-	expression tag
Pro 861	-	expression tag
Gln 862	-	expression tag
Phe 863	-	expression tag
Glu 864	-	expression tag
Lys 865	-	expression tag
Asp 866	-	expression tag
Tyr 867	-	expression tag
Lys 868	-	expression tag
Asp 869	-	expression tag
Asp 870	-	expression tag
Asp 871	-	expression tag
Asp 872	-	expression tag
Lys 873	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	130701.9
All*	Total formula weight	130701.9

*Water molecules are not included.