7WTT
Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A1 (with CK1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (2) | 18S rRNA | polymer | 1873 | 604117.8 | 1 | Homo sapiens | ||
| 2 | B (R) | 40S ribosomal protein S17 | polymer | 135 | 15578.2 | 1 | UniProt (P08708) Pfam (PF00833) | Homo sapiens (human) | Small ribosomal subunit protein eS17 |
| 3 | C (b) | 40S ribosomal protein S27 | polymer | 84 | 9480.2 | 1 | UniProt (P42677) Pfam (PF01667) | Homo sapiens (human) | Metallopan-stimulin 1,MPS-1,Small ribosomal subunit protein eS27 |
| 4 | D (B) | 40S ribosomal protein S3a | polymer | 264 | 30002.1 | 1 | UniProt (P61247) Pfam (PF01015) | Homo sapiens (human) | Small ribosomal subunit protein eS1,v-fos transformation effector protein,Fte-1 |
| 5 | E (c) | 40S ribosomal protein S28 | polymer | 69 | 7855.1 | 1 | UniProt (P62857) Pfam (PF01200) | Homo sapiens (human) | Small ribosomal subunit protein eS28 |
| 6 | F (E) | 40S ribosomal protein S4, X isoform | polymer | 263 | 29654.9 | 1 | UniProt (P62701) Pfam (PF08071) Pfam (PF00900) Pfam (PF00467) Pfam (PF16121) | Homo sapiens (human) | SCR10,Single copy abundant mRNA protein,Small ribosomal subunit protein eS4 |
| 7 | G (e) | 40S ribosomal protein S30 | polymer | 59 | 6668.9 | 1 | UniProt (P62861) Pfam (PF04758) | Homo sapiens (human) | Small ribosomal subunit protein eS30 |
| 8 | H (F) | 40S ribosomal protein S5 | polymer | 204 | 22913.5 | 1 | UniProt (P46782) Pfam (PF00177) | Homo sapiens (human) | Small ribosomal subunit protein uS7 |
| 9 | I (H) | 40S ribosomal protein S7 | polymer | 194 | 22168.9 | 1 | UniProt (P62081) Pfam (PF01251) | Homo sapiens (human) | Small ribosomal subunit protein eS7 |
| 10 | J (G) | 40S ribosomal protein S6 | polymer | 249 | 28751.9 | 1 | UniProt (P62753) Pfam (PF01092) | Homo sapiens (human) | Phosphoprotein NP33,Small ribosomal subunit protein eS6 |
| 11 | K (Z) | 40S ribosomal protein S25 | polymer | 125 | 13776.2 | 1 | UniProt (P62851) Pfam (PF03297) | Homo sapiens (human) | Small ribosomal subunit protein eS25 |
| 12 | L (Y) | 40S ribosomal protein S24 | polymer | 133 | 15463.3 | 1 | UniProt (P62847) Pfam (PF01282) | Homo sapiens (human) | Small ribosomal subunit protein eS24 |
| 13 | M (x) | RNA-binding protein PNO1 | polymer | 252 | 27970.4 | 1 | UniProt (Q9NRX1) Pfam (PF22891) | Homo sapiens (human) | Partner of NOB1 |
| 14 | N (X) | 40S ribosomal protein S23 | polymer | 143 | 15844.7 | 1 | UniProt (P62266) Pfam (PF00164) | Homo sapiens (human) | Small ribosomal subunit protein uS12 |
| 15 | O (w) | Bystin | polymer | 437 | 49673.3 | 1 | UniProt (Q13895) Pfam (PF05291) | Homo sapiens (human) | |
| 16 | P (W) | 40S ribosomal protein S15a | polymer | 130 | 14865.6 | 1 | UniProt (P62244) Pfam (PF00410) | Homo sapiens (human) | Small ribosomal subunit protein uS8 |
| 17 | Q (u) | Pre-rRNA-processing protein TSR1 homolog | polymer | 804 | 91951.2 | 1 | UniProt (Q2NL82) Pfam (PF22298) Pfam (PF08142) Pfam (PF04950) | Homo sapiens (human) | |
| 18 | R (t) | Protein LTV1 homolog | polymer | 475 | 54935.9 | 1 | UniProt (Q96GA3) Pfam (PF04180) | Homo sapiens (human) | |
| 19 | S (T) | 40S ribosomal protein S19 | polymer | 145 | 16091.6 | 1 | UniProt (P39019) Pfam (PF01090) | Homo sapiens (human) | Small ribosomal subunit protein eS19 |
| 20 | T (S) | 40S ribosomal protein S18 | polymer | 152 | 17759.8 | 1 | UniProt (P62269) Pfam (PF00416) | Homo sapiens (human) | Ke-3,Ke3,Small ribosomal subunit protein uS13 |
| 21 | U (Q) | 40S ribosomal protein S16 | polymer | 146 | 16477.4 | 1 | UniProt (P62249) Pfam (PF00380) | Homo sapiens (human) | Small ribosomal subunit protein uS9 |
| 22 | V (P) | 40S ribosomal protein S15 | polymer | 145 | 17076.2 | 1 | UniProt (P62841) Pfam (PF00203) | Homo sapiens (human) | RIG protein,Small ribosomal subunit protein uS19 |
| 23 | W (O) | 40S ribosomal protein S14 | polymer | 151 | 16302.8 | 1 | UniProt (P62263) Pfam (PF00411) | Homo sapiens (human) | Small ribosomal subunit protein uS11 |
| 24 | X (N) | 40S ribosomal protein S13 | polymer | 151 | 17259.4 | 1 | UniProt (P62277) Pfam (PF08069) Pfam (PF00312) | Homo sapiens (human) | Small ribosomal subunit protein uS15 |
| 25 | Y (L) | 40S ribosomal protein S11 | polymer | 158 | 18468.8 | 1 | UniProt (P62280) Pfam (PF16205) Pfam (PF00366) | Homo sapiens (human) | Small ribosomal subunit protein uS17 |
| 26 | Z (J) | 40S ribosomal protein S9 | polymer | 194 | 22641.6 | 1 | UniProt (P46781) Pfam (PF00163) Pfam (PF01479) | Homo sapiens (human) | Small ribosomal subunit protein uS4 |
| 27 | AA (I) | 40S ribosomal protein S8 | polymer | 208 | 24263.4 | 1 | UniProt (P62241) Pfam (PF01201) | Homo sapiens (human) | Small ribosomal subunit protein eS8 |
| 28 | BA (r) | Multifunctional methyltransferase subunit TRM112-like protein | polymer | 125 | 14215.4 | 1 | UniProt (Q9UI30) Pfam (PF03966) | Homo sapiens (human) | |
| 29 | CA (q) | Probable 18S rRNA (guanine-N(7))-methyltransferase | polymer | 281 | 31925.4 | 1 | UniProt (O43709) Pfam (PF08241) Pfam (PF12589) | Homo sapiens (human) | Bud site selection protein 23 homolog,Metastasis-related methyltransferase 1,Williams-Beuren syndrome chromosomal region 22 protein,rRNA methyltransferase and ribosome maturation factor |
| 30 | DA (K) | RRP12-like protein | polymer | 1297 | 143916.5 | 1 | UniProt (Q5JTH9) Pfam (PF08161) | Homo sapiens (human) | |
| 31 | EA (M) | 40S ribosomal protein S12 | polymer | 132 | 14539.0 | 1 | UniProt (P25398) Pfam (PF01248) | Homo sapiens (human) | Small ribosomal subunit protein eS12 |
| 32 | FA (f) | Ubiquitin-40S ribosomal protein S27a | polymer | 156 | 18004.0 | 1 | UniProt (P62979) Pfam (PF00240) Pfam (PF01599) | Homo sapiens (human) | Ubiquitin carboxyl extension protein 80 |
| 33 | GA (a) | Casein kinase I isoform alpha | polymer | 337 | 38944.8 | 1 | UniProt (P48729) Pfam (PF00069) | Homo sapiens (human) | CKI-alpha,CK1 |
| 34 | HA (z) | Ribosome biogenesis protein SLX9 homolog | polymer | 230 | 25503.3 | 1 | UniProt (Q9NSI2) Pfam (PF15341) | Homo sapiens (human) | |
| 35 | IA (q) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 36 | JA (f) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
a: 1 - 337 (UniProt: P48729)
| PDB | External Database | Details |
|---|---|---|
| Gly 20 | Val 20 | conflict |
| Thr 35 | Ile 35 | conflict |
| His 58 | Leu 58 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 34 |
| Total formula weight | 1515061.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 449.8 | |
| All* | Total formula weight | 1515510.9 |
