7WQ3

Galanin-bound galanin receptor 1 in complex with Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Galanin receptor type 1	polymer	349	38999.8	1	UniProt (P47211) Pfam (PF00001) In PDB	Homo sapiens (human)	GAL1-R,GALR-1
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus	Transducin beta chain 1
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
5	S	ScFv16	polymer	247	26277.3	1		Mus musculus
6	L	Galanin	polymer	30	3161.4	1	UniProt (P22466) Pfam (PF01296) In PDB	Homo sapiens
7	R	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	155458.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1933.3
All*	Total formula weight	157391.3

*Water molecules are not included.