7WNW

Crystal structure of Imine Reductase Mutant(M5) from Actinoalloteichus hymeniacidonis in complex with NADPH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	3-hydroxyisobutyrate dehydrogenase-like beta-hydroxyacid dehydrogenase	polymer	336	35260.7	2	UniProt (A0A1D8BXU6)	Actinoalloteichus hymeniacidonis	Imine Reductase
2	C, D (B, A)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
3	E, F (B, A)	water	water		18.0	251	Chemie (HOH)

Sequence modifications

B, A: 1 - 316 (UniProt: A0A1D8BXU6)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Thr 101	Asn 101	engineered mutation
Lys 125	Gly 125	engineered mutation
Ala 169	His 169	engineered mutation
Ile 177	Leu 177	engineered mutation
Ile 187	Phe 187	engineered mutation
Ala 218	Met 218	engineered mutation
Phe 240	Ser 240	engineered mutation
His 244	Met 244	engineered mutation
His 247	Ala 247	engineered mutation
Lys 251	Asn 251	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70521.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1486.8
All*	Total formula weight	72008.1

*Water molecules are not included.