7WJF

Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase mutant D394A in complex with kojibiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha-xylosidase	polymer	759	87984.5	1	UniProt (A2RM80) Pfam (PF01055) Pfam (PF21365)	Lactococcus lactis subsp. cremoris MG1363
2	B (B)	alpha-D-glucopyranose-(1-2)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G46435VD)
3	C, D, E, F, G... (A)	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
4	K (A)	water	water		18.0	580	Chemie (HOH)

Sequence modifications

A: 1 - 739 (UniProt: A2RM80)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 394	Asp 394	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	87984.5
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	8
	Total formula weight	496.5
All*	Total formula weight	88823.3

*Water molecules are not included.