7WJE
Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase mutant D394A in complex with nigerotetraose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Alpha-xylosidase | polymer | 759 | 87984.5 | 1 | UniProt (A2RM80) Pfam (PF01055) Pfam (PF21365) In PDB | Lactococcus lactis subsp. cremoris MG1363 | |
2 | B | alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose-(1-3)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB GlyTouCan (G72266UI) | |||
3 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
4 | water | water | 18.0 | 638 | Chemie (HOH) |
Sequence modifications
A: 1 - 739 (UniProt: A2RM80)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 394 | Asp 394 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 87984.5 | |
Branched | Number of molecules | 1 |
Total formula weight | 666.6 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 310.3 | |
All* | Total formula weight | 88961.4 |