7WH6

The mutant crystal structure of b-1,4-Xylanase (XynAF1_N179S)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-xylanase	polymer	329	35213.2	2	UniProt (A0A229WLM4) Pfam (PF00331) UniProt (by SIFTS) (Q4WLG5) In PDB	Aspergillus fumigatus (Neosartorya fumigata)
2	C	alpha-D-mannopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G97616MU)
3	D	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G42227JK)
4	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5		water	water		18.0	827	Chemie (HOH)

Sequence modifications

A, B: 5 - 329 (UniProt: A0A229WLM4)

PDB	External Database	Details
Glu 1	-	expression tag
Ala 2	-	expression tag
Glu 3	-	expression tag
Ala 4	-	expression tag
Ser 179	Asn 194	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70426.3
Branched	Number of molecules	2
	Total formula weight	2145.9
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	72756.5

*Water molecules are not included.