7WCM

Cryo-EM structure of GPR119-Gs Complex with small molecule agonist MBX-2982

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
4	N	Nb35	polymer	138	15140.7	1		Lama glama
5	R	Glucose-dependent insulinotropic receptor	polymer	335	36936.7	1	UniProt (Q8TDV5) Pfam (PF00001) In PDB	Homo sapiens (human)	G-protein coupled receptor 119
6	R	2-[1-(5-ethylpyrimidin-2-yl)piperidin-4-yl]-4-[[4-(1,2,3,4-tetrazol-1-yl)phenoxy]methyl]-1,3-thiazole	non-polymer		448.5	1	Chemie (8VP)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 13 - 351 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Leu 10	-	expression tag
Leu 11	-	expression tag
Gln 12	-	expression tag

R: 1 - 335 (UniProt: Q8TDV5)

PDB	External Database	Details
Cys 237	Ser 237	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	144366.4
Non-Polymers*	Number of molecules	1
	Total formula weight	448.5
All*	Total formula weight	144815.0

*Water molecules are not included.