7WBJ

Cryo-EM structure of N-terminal modified human vasoactive intestinal polypeptide receptor 2 (VIP2R) in complex with PACAP27 and Gs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Vasoactive intestinal polypeptide receptor 2	polymer	432	49172.7	1	UniProt (P41587) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	VIP-R-2,Helodermin-preferring VIP receptor,Pituitary adenylate cyclase-activating polypeptide type III receptor,PACAP type III receptor,PACAP-R-3,PACAP-R3,VPAC2
2	B (L)	Pituitary adenylate cyclase-activating polypeptide 27	polymer	27	3154.6	1	UniProt (P18509) Pfam (PF00123)	Homo sapiens	PACAP-27,PACAP27
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45743.4	1	UniProt (P04896) Pfam (PF00503)	Bos taurus (cattle)	Adenylate cyclase-stimulating G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	400	43706.8	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (cattle)	G gamma-I
6	F (N)	Nanobody-35	polymer	140	15343.0	1		synthetic construct

Sequence modifications

R: 24 - 438 (UniProt: P41587)

PDB	External Database	Details
Asp 7	-	expression tag
Tyr 8	-	expression tag
Lys 9	-	expression tag
Asp 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Val 14	-	expression tag
Asp 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag

A: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation
Ser 366	Ala 366	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -33	-	initiating methionine
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	164981.7
All*	Total formula weight	164981.7

*Water molecules are not included.