7WAN
Crystal structure of HaloTag complexed with UL2
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Haloalkane dehalogenase | polymer | 299 | 33716.4 | 1 | UniProt (P0A3G3) Pfam (PF00561) In PDB | Rhodococcus sp. | |
2 | A | (R)-[4-(2-azanylhydrazinyl)phenyl]-[2-[2-(2-hexoxyethoxy)ethoxy]ethylamino]methanol | non-polymer | 384.5 | 1 | Chemie (8MS) | |||
3 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
4 | water | water | 18.0 | 96 | Chemie (HOH) |
Sequence modifications
A: 1 - 292 (UniProt: P0A3G3)
PDB | External Database | Details |
---|---|---|
Ser -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Ala 1 | Ser 2 | engineered mutation |
Val 46 | Leu 47 | engineered mutation |
Thr 57 | Ser 58 | engineered mutation |
Gly 77 | Asp 78 | engineered mutation |
Phe 86 | Tyr 87 | engineered mutation |
Met 87 | Leu 88 | engineered mutation |
Phe 127 | Cys 128 | engineered mutation |
Thr 154 | Ala 155 | engineered mutation |
Lys 159 | Glu 160 | engineered mutation |
Val 166 | Ala 167 | engineered mutation |
Thr 171 | Ala 172 | engineered mutation |
Cys 174 | Lys 175 | engineered mutation |
Gly 175 | Cys 176 | engineered mutation |
Asn 194 | Lys 195 | engineered mutation |
Glu 223 | Ala 224 | engineered mutation |
Asp 226 | Asn 227 | engineered mutation |
Lys 256 | Glu 257 | engineered mutation |
Ala 263 | Thr 264 | engineered mutation |
Asn 271 | His 272 | engineered mutation |
Leu 272 | Tyr 273 | engineered mutation |
Ser 290 | Pro 291 | engineered mutation |
Thr 291 | Ala 292 | engineered mutation |
Glu 293 | - | expression tag |
Ile 294 | - | expression tag |
Ser 295 | - | expression tag |
Gly 296 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 33716.4 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 420.0 | |
All* | Total formula weight | 34136.4 |