7W9V

Cryo-EM structure of nucleosome in complex with p300 acetyltransferase catalytic core (complex I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.1	polymer	135	15306.0	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F (B, F)	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I (I)	DNA (145-MER)	polymer	145	44520.4	1		synthetic construct
6	J (J)	DNA (145-MER)	polymer	145	44991.7	1		synthetic construct
7	K (K)	Histone acetyltransferase p300	polymer	638	74426.6	1	UniProt (Q09472) Pfam (PF00569) Pfam (PF00439) Pfam (PF06001) Pfam (PF23570) Pfam (PF08214)	Homo sapiens (human)	p300 HAT,E1A-associated protein p300,Histone butyryltransferase p300,Histone crotonyltransferase p300,Protein 2-hydroxyisobutyryltransferase p300,Protein lactyltransferas p300,Protein propionyltransferase p300

Sequence modifications

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

K: 1035 - 1519 (UniProt: Q09472)

PDB	External Database	Details
Phe 1467	Tyr 1467	engineered mutation

K: 1581 - 1720 (UniProt: Q09472)

PDB	External Database	Details
Ser 1520	-	linker
Gly 1521	-	linker
Gly 1522	-	linker
Ser 1523	-	linker
Gly 1580	-	linker
His 1721	-	expression tag
His 1722	-	expression tag
His 1723	-	expression tag
His 1724	-	expression tag
His 1725	-	expression tag
His 1726	-	expression tag
His 1727	-	expression tag
His 1728	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	275234.6
All*	Total formula weight	275234.6

*Water molecules are not included.