7W8E

Sweet taste protein Brazzein - R43A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Defensin-like protein	polymer	69	8263.4	1	UniProt (P56552) In PDB	Pentadiplandra brazzeana	Brazzein
2	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
3		water	water		18.0	49	Chemie (HOH)

Sequence modifications

A: 1 - 54 (UniProt: P56552)

PDB	External Database	Details
Met -5	-	initiating methionine
Ala -4	-	expression tag
Asn -3	-	expression tag
Pro -2	-	expression tag
Arg -1	-	expression tag
Val 0	-	expression tag
Met 1	Gln 1	conflict
Ala 43	Arg 43	engineered mutation
Pro 55	-	expression tag
Leu 56	-	expression tag
Glu 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	8263.4
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	8286.4

*Water molecules are not included.