7W6L

The crystal structure of MLL3-RBBP5-ASH2L in complex with H3K4me0 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Set1/Ash2 histone methyltransferase complex subunit ASH2	polymer	184	20597.4	2	UniProt (Q9UBL3) Pfam (PF00622)	Homo sapiens (human)	ASH2-like protein
2	C, E (C, E)	Histone-lysine N-methyltransferase 2C	polymer	159	18442.1	2	UniProt (Q8NEZ4) Pfam (PF00856)	Homo sapiens (human)	Lysine N-methyltransferase 2C,Homologous to ALR protein,Myeloid/lymphoid or mixed-lineage leukemia protein 3
3	D, F (D, F)	Retinoblastoma-binding protein 5	polymer	27	3255.3	2	UniProt (Q15291)	Homo sapiens (human)	RBBP-5,Retinoblastoma-binding protein RBQ-3
4	G (M)	Histone H3.3C	polymer	9	1063.3	1	UniProt (Q6NXT2)	Homo sapiens	H3K4me0 peptide
5	H, J (C, E)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	I, K (C, E)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
7	L, M, N, O, P... (A, B, C, D, E...)	water	water		18.0	225	Chemie (HOH)

Sequence modifications

A, B: 286 - 438 (UniProt: Q9UBL3)

PDB	External Database	Details
Ser 285	-	expression tag

A, B: 445 - 504 (UniProt: Q9UBL3)

PDB	External Database	Details
Ile 439	-	linker
Ser 440	-	linker
Gly 441	-	linker
Arg 442	-	linker
Gly 443	-	linker
Ser 444	-	linker

C, E: 4757 - 4911 (UniProt: Q8NEZ4)

PDB	External Database	Details
Gly 4753	-	expression tag
Pro 4754	-	expression tag
Leu 4755	-	expression tag
Gly 4756	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	85652.8
Non-Polymers*	Number of molecules	4
	Total formula weight	899.6
All*	Total formula weight	86552.4

*Water molecules are not included.