7W5X

Cryo-EM structure of SoxS-dependent transcription activation complex with zwf promoter DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (1)	zwf promoter DNA forward strand	polymer	75	23298.9	1		Escherichia coli
2	B (2)	zwf promoter DNA reverse strand	polymer	75	23120.8	1		Escherichia coli
3	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150804.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
4	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli K-12	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
5	E (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
6	F (F)	RNA polymerase sigma factor RpoD	polymer	613	70352.2	1	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Escherichia coli K-12	Sigma-70
7	G (K)	Regulatory protein SoxS	polymer	107	12931.8	1	UniProt (P0A9E2) Pfam (PF00165)	Escherichia coli K-12
8	H, I (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli K-12	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha

Sequence modifications

C: 1 - 1342 (UniProt: P0A8V2)

PDB	External Database	Details
Val 516	Asp 516	variant

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	519242.4
All*	Total formula weight	519242.4

*Water molecules are not included.