7W5W
Cryo-EM structure of SoxS-dependent transcription activation complex with micF promoter DNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150804.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | F | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli K-12 | Sigma-70 |
6 | 1 | micF promoter DNA forward strand | polymer | 70 | 21574.9 | 1 | Escherichia coli | ||
7 | 2 | micF promoter DNA reverse strand | polymer | 70 | 21751.9 | 1 | Escherichia coli | ||
8 | J | Regulatory protein SoxS | polymer | 107 | 12931.8 | 1 | UniProt (P0A9E2) Pfam (PF00165) In PDB | Escherichia coli K-12 | |
9 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
10 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
C: 1 - 1342 (UniProt: P0A8V2)
PDB | External Database | Details |
---|---|---|
Val 516 | Asp 516 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 516149.5 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 516304.6 |