7W57

Cryo-EM structure of the neuromedin S-bound neuromedin U receptor 2-Gq protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	361	41724.4	1	Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) In PDB	Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Neuromedin-S	polymer	34	3927.5	1	UniProt (Q5H8A3) In PDB	Homo sapiens (human)
4	E	scFv16	polymer	248	26496.5	1		Homo sapiens
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
6	R	Neuromedin-U receptor 2	polymer	571	65417.4	1	Pfam (PF07361) Pfam (PF00001) Pfam (PF19285) UniProt (by SIFTS) (P0ABE7) UniProt (by SIFTS) (Q9GZQ4) In PDB	Homo sapiens (human)

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

C: -23 - 9 (UniProt: Q5H8A3)

PDB	External Database	Details
Gln 10	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	183211.2
All*	Total formula weight	183211.2

*Water molecules are not included.