7W2Z

Cryo-EM structure of the ghrelin-bound human ghrelin receptor-Go complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	236	26456.1	1	UniProt (A0A1W2PS82) UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
2	B (R)	Growth hormone secretagogue receptor type 1	polymer	366	41364.3	1	UniProt (Q92847) Pfam (PF00001)	Homo sapiens (human)	GHS-R,GH-releasing peptide receptor,GHRP,Ghrelin receptor
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	ScFv16	polymer	250	26610.6	1		synthetic construct
5	E (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
6	F (L)	Appetite-regulating hormone	polymer	16	1998.3	1	UniProt (Q9UBU3) Pfam (PF04644)	Homo sapiens (human)
7	G, H (R)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

A: 4 - 56 (UniProt: A0A1W2PS82)

PDB	External Database	Details
Met 3	-	initiating methionine
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation
His 173	-	linker
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker

A: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

L: 1 - 16 (UniProt: Q9UBU3)

PDB	External Database	Details
K4q 3	Ser 26	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	141576.2
Non-Polymers*	Number of molecules	2
	Total formula weight	773.3
All*	Total formula weight	142349.5

*Water molecules are not included.