7W2Z
Cryo-EM structure of the ghrelin-bound human ghrelin receptor-Go complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 236 | 26456.1 | 1 | UniProt (A0A1W2PS82) UniProt (P09471) Pfam (PF00503) | Homo sapiens (human) | |
| 2 | B (R) | Growth hormone secretagogue receptor type 1 | polymer | 366 | 41364.3 | 1 | UniProt (Q92847) Pfam (PF00001) | Homo sapiens (human) | GHS-R,GH-releasing peptide receptor,GHRP,Ghrelin receptor |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (S) | ScFv16 | polymer | 250 | 26610.6 | 1 | synthetic construct | ||
| 5 | E (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 6 | F (L) | Appetite-regulating hormone | polymer | 16 | 1998.3 | 1 | UniProt (Q9UBU3) Pfam (PF04644) | Homo sapiens (human) | |
| 7 | G, H (R) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) PubChem (16219173) PubChem (16758052) PubChem (176980071) PubChem (18640088) PubChem (1715243) PubChem (1715244) PubChem (23423981) PubChem (44654663) PubChem (46937002) PubChem (40477506) PubChem (40557614) PubChem (42579431) PubChem (42579432) PubChem (59274407) PubChem (45044259) PubChem (68311425) PubChem (68311398) PubChem (143173313) PubChem (177156123) PubChem (5997) PubChem (1551269) PubChem (90070115) PubChem (49853509) PubChem (50990013) PubChem (117965027) PubChem (6432564) PubChem (6710666) PubChem (124146240) PubChem (52934537) PubChem (7059399) PubChem (124897607) PubChem (124896959) PubChem (10861996) PubChem (56621683) PubChem (132837164) PubChem (11834483) PubChem (11888915) PubChem (11888914) PubChem (138967089) PubChem (139566851) PubChem (15939944) PubChem (57915018) PubChem (57939063) PubChem (58428240) PubChem (58445498) PubChem (58663286) PubChem (171828367) PubChem (173516975) PubChem (59450329) PubChem (59628412) PubChem (59965215) PubChem (71234641) PubChem (59993755) PubChem (60001463) PubChem (66634651) PubChem (68055877) PubChem (68166463) PubChem (68315613) PubChem (70795838) PubChem (70797612) PubChem (70954610) PubChem (70957185) PubChem (71463909) PubChem (89951918) PubChem (90224410) PubChem (91298236) PubChem (101624109) PubChem (304) PubChem (124225449) PubChem (191463) PubChem (368063) PubChem (369319) PubChem (124429284) PubChem (638019) PubChem (124909223) PubChem (124909224) PubChem (124909225) PubChem (1549081) PubChem (1553803) PubChem (5283629) PubChem (5284644) PubChem (5315842) PubChem (6419195) PubChem (6994560) PubChem (7055705) PubChem (7055706) PubChem (7059398) PubChem (7059400) PubChem (7059635) PubChem (7103033) PubChem (7182477) PubChem (7182478) PubChem (9909206) PubChem (10045721) PubChem (71168115) PubChem (10134774) PubChem (10293553) PubChem (71173031) PubChem (11003580) PubChem (11025495) PubChem (11058155) PubChem (11463329) PubChem (11868624) PubChem (11868625) PubChem (11869366) PubChem (11869367) PubChem (11869368) PubChem (11869369) PubChem (130459183) PubChem (12120344) PubChem (12156156) PubChem (12303135) PubChem (12303148) PubChem (12303156) PubChem (12303159) PubChem (12303160) PubChem (131992107) PubChem (139568882) PubChem (142983773) PubChem (144054483) PubChem (144209461) PubChem (144331236) PubChem (144808354) PubChem (145053568) PubChem (145157652) PubChem (145215073) PubChem (145230478) PubChem (146320991) PubChem (147857230) PubChem (154993519) PubChem (158603858) PubChem (162906452) PubChem (162906453) PubChem (162906454) PubChem (162906455) PubChem (162906456) PubChem (162906457) PubChem (163255768) PubChem (163389210) PubChem (164665303) PubChem (166553771) PubChem (166712004) PubChem (170116175) PubChem (171764280) PubChem (171817101) PubChem (171817104) PubChem (174285573) |
Sequence modifications
A: 4 - 56 (UniProt: A0A1W2PS82)
A: 182 - 354 (UniProt: P09471)
L: 1 - 16 (UniProt: Q9UBU3)
| PDB | External Database | Details |
|---|---|---|
| Met 3 | - | initiating methionine |
| Asp 42 | Gly 42 | engineered mutation |
| Asn 43 | Glu 43 | engineered mutation |
| His 173 | - | linker |
| Gly 174 | - | linker |
| Gly 175 | - | linker |
| Ser 176 | - | linker |
| Gly 177 | - | linker |
| Gly 178 | - | linker |
| Ser 179 | - | linker |
| Gly 180 | - | linker |
| Gly 181 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asp 227 | Ala 227 | engineered mutation |
| Asp 230 | Gly 230 | engineered mutation |
| Ala 332 | Ile 332 | engineered mutation |
| Ile 335 | Val 335 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| K4q 3 | Ser 26 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 141576.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 773.3 | |
| All* | Total formula weight | 142349.5 |
