7W2Z
Cryo-EM structure of the ghrelin-bound human ghrelin receptor-Go complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(o) subunit alpha | polymer | 236 | 26456.1 | 1 | UniProt (A0A1W2PS82) UniProt (P09471) Pfam (PF00503) In PDB | Homo sapiens (human) | |
2 | R | Growth hormone secretagogue receptor type 1 | polymer | 366 | 41364.3 | 1 | UniProt (Q92847) Pfam (PF00001) In PDB | Homo sapiens (human) | GHS-R,GH-releasing peptide receptor,GHRP,Ghrelin receptor |
3 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
4 | S | ScFv16 | polymer | 250 | 26610.6 | 1 | synthetic construct | ||
5 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
6 | L | Appetite-regulating hormone | polymer | 16 | 1998.3 | 1 | UniProt (Q9UBU3) Pfam (PF04644) In PDB | Homo sapiens (human) | |
7 | R | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) |
Sequence modifications
A: 4 - 56 (UniProt: A0A1W2PS82)
A: 182 - 354 (UniProt: P09471)
L: 1 - 16 (UniProt: Q9UBU3)
PDB | External Database | Details |
---|---|---|
Met 3 | - | initiating methionine |
Asp 42 | Gly 42 | engineered mutation |
Asn 43 | Glu 43 | engineered mutation |
His 173 | - | linker |
Gly 174 | - | linker |
Gly 175 | - | linker |
Ser 176 | - | linker |
Gly 177 | - | linker |
Gly 178 | - | linker |
Ser 179 | - | linker |
Gly 180 | - | linker |
Gly 181 | - | linker |
PDB | External Database | Details |
---|---|---|
Asp 227 | Ala 227 | engineered mutation |
Asp 230 | Gly 230 | engineered mutation |
Ala 332 | Ile 332 | engineered mutation |
Ile 335 | Val 335 | engineered mutation |
PDB | External Database | Details |
---|---|---|
K4q 3 | Ser 26 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 141576.2 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 773.3 | |
All* | Total formula weight | 142349.5 |