7W1N

Complex structure of a leaf-branch compost cutinase variant LCC ICCG_KRP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leaf-branch compost cutinase	polymer	270	28592.0	1	UniProt (G9BY57) Pfam (PF12695)	Unknown prokaryotic organism	LC-cutinase,LCC,PET-digesting enzyme,Poly(ethylene terephthalate) hydrolase,PET hydrolase,PETase
2	B (A)	BICINE	non-polymer		163.2	1	Chemie (BCN)
3	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	D (A)	water	water		18.0	330	Chemie (HOH)

Sequence modifications

A: 36 - 293 (UniProt: G9BY57)

PDB	External Database	Details
Gly 24	-	expression tag
Ala 25	-	expression tag
Gly 26	-	expression tag
Ala 27	-	expression tag
Gly 28	-	expression tag
Ala 29	-	expression tag
Gly 30	-	expression tag
Ala 31	-	expression tag
Gly 32	-	expression tag
Ala 33	-	expression tag
Gly 34	-	expression tag
Met 35	-	expression tag
Lys 59	Ala 59	engineered mutation
Arg 75	Val 75	engineered mutation
Gly 127	Tyr 127	engineered mutation
Cys 238	Asp 238	engineered mutation
Ile 243	Phe 243	engineered mutation
Pro 248	Asn 248	engineered mutation
Cys 283	Ser 283	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28592.0
Non-Polymers*	Number of molecules	2
	Total formula weight	225.2
All*	Total formula weight	28817.3

*Water molecules are not included.