7VZQ

The structure of GdmN V24Y/G157A/R158A/G188R mutant in complex with carbamoyl adenylate intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GdmN	polymer	702	76629.1	2	UniProt (Q84G19) Pfam (PF02543) Pfam (PF16861)	Streptomyces hygroscopicus
2	C (A)	PHOSPHORIC ACID MONO(FORMAMIDE)ESTER	non-polymer		141.0	1	Chemie (CP)
3	D, O (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
4	E, F, G, H, P... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
5	I, R (A, B)	5'-O-[(S)-(carbamoyloxy)(hydroxy)phosphoryl]adenosine	non-polymer		390.2	2	Chemie (CA0)
6	J, K, L, M, N... (A, B)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
7	V (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	W, X (A, B)	water	water		18.0	261	Chemie (HOH)

Sequence modifications

A, B: 1 - 682 (UniProt: Q84G19)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Tyr 24	Val 24	engineered mutation
Ala 157	Gly 157	engineered mutation
Ala 158	Arg 158	engineered mutation
Arg 188	Gly 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	153258.2
Non-Polymers*	Number of molecules	20
	Total formula weight	2209.6
All*	Total formula weight	155467.7

*Water molecules are not included.